What It Does
Advanced Solutions Life Sciences patented and patent pending vascularization technologies provide a flexible, customizable, and effective approaches at integrating microvasculatures for in vitro assay, tissue fabrication, vascular model, and a variety of other applications.
Available as three different, vascularization product lines, our Microvessel Platform, In vitro Perfusion Module (IPM™), and Vascularized IPM™ (VIPM™) represent a complete vascularization solution addressing the need of a wide-spectrum of applications. The ability to recapitulate a microvascular perfusion in vitro, via the integration of rapidly fabricated microfluidic channels (IPM) and native microvasculatures (VIPM), has a significant impact in a number of areas. For example, in drug and therapeutics discovery and development, a vascularized tissue mimic with perfusion profoundly changes the predictive ability of screening assays. Additionally, incorporation of a perfused, functional microvasculature (Microvessel Platform), which is capable of adapting to match tissue-specific functionality, into tissue fabrication protocols fully enables the promise of regenerative medicine and tissue replacement approaches.
How it Works
Microvessel technology: Our microvessel technology is a vascularization platform involving fresh isolates of adipose-derived, whole microvessels, which contain all vascular types maintained in the native microvessel structure and capable of recapitulating a stable, adaptive microvasculature. When placed in 3D matrices, the microvessels can recapitulate angiogenesis, forming neovessels that fill the matrix. When perfused, the neovessels adapt and remodel to form a mature microcirculation. This flexible and adaptive platform has proven effective as a research model of angiogenesis, an in vitro assay platform, an effective vascularization approach in tissue fabrication (including bioprinting), and in the rescue of ischemic insult in preclinical models. All of this past and ongoing work demonstrates the utility, flexibility, and versatility of this microvessel system in either in vitro or in vivo applications.
IPM/VIPM: The IPM™ device entails an array of microfluidic channels of pre-determined dimensions incorporated into a biocompatible housing unit designed to accommodate a region of living cell systems directly perfused via the endothelial cell lined channels. Importantly, fabrication of the device involves an “all-in-one” strategy in which the channels, perfusion ports, and device structure are-3D printed in a single, integrated print job; a functionality and workflow unique to the BioAssemblyBot® bioprinter. Advanced Solutions Life Sciences isolated human, intact microvessels are integrated into the channelized construct, enabling the channels and the microvessels to connect or inosculate within the matrix environment surrounding the channels. After inosculation occurs, the native microvasculature can adapt and mature based on hemodynamic cues provided by the microfluidic platform enabling a VIPM™.